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KMID : 0894520120160040289
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Development & Reproduction
2012 Volume.16 No. 4 p.289 ~ p.294
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Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica
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Choi Min-Seop
Kwon Se-Ryun
Kwon Hyuk-Chu
Choi Seong-Hee
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Abstract
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Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[a]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-17B (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P (10^{-6}-10^{-5}M) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT (10^{-9}-10^{-5}M) the gene expressions of CYP1A and AhR were suppressed at high concentrations (10^{-6}-10^{-5}M), while having no effects at low concentrations (10^{-9}-10^{-7}M). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-17B.
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KEYWORD
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Eel hepatocytes, B[a]P, TBT, CYP1A, AhR, Vitellogenin
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